Artificial incubation and hatching of embryos of the red swamp crayfish <i>Procambarus clarkii</i> (Decapoda: Cambaridae)

We studied the artificial incubation method of red swamp crayfish Procambarus clarkii embryos using cell culture plates. In first experiment, cleavage stage or egg-nauplius were individually cultured at 20°C in well plates with different water volumes per [12 (4 ml), 24 (2 48 (1 ml) and 96 (0.25 ml)]. second 12-well 15, 20 25°C. Almost all died without hatching. Egg-nauplius survived to hatch. 96-well plates, embryo hatched, but instar juveniles soon. other mortality tended increase decreasing volume; particularly, high was recorded 48-well Hatching moulting rates 15°C 20°C, approximately half concluded that a plate ml well) is suitable for incubating P. hatch moult 15–20°C.